Monthly Archives: February 2012

Wednesday 02/29/12

Posted on February 29, 2012 by admin

9:45 A — 11:00P Washing out O/N 4C antibodies from new protein stains Flip Fly stocks Flip vials and enter new GFP lines into fly stocks. Start expanding Gal4 bcd-3′ UTR (to try crossing into PcG RNAi lines.  will need … Continue reading →

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STORM 2-28-11

Posted on February 29, 2012 by admin

Laser powers: 750: 104 mW 647 : 150 mW 561: 150 mW 488: 150 mW 405: 1.5 mW Sample 2H.  750 switching nicely. 647 very bright conventional images.  Under high laser power image still inverts — low nuc singal high … Continue reading →

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Tuesday 02/28/12

Posted on February 28, 2012 by admin

9:45 A – 12:45 A Evaluating 3D fitting: section joins do not look smooth Wrote new script (list2tif3d_weq.m) which distributes samples uniformly in z.  Samples near the focal plane are best resolved and can have higher z-resolution. Current approach still … Continue reading →

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Monday 02/27/12

Posted on February 27, 2012 by admin

9:40 A – 7:40P, 8:30P – 12:15P Setup sample S2 33o nm (4 color) and Psc-hox 330 nm (2 color) to run through Insight. Sectioning: 2 slides of 2H and 2 slides of rad21 cut.  slides 2H react strongly to … Continue reading →

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Protected: Super-resolution imaging of PcG bodies

Posted on February 27, 2012 by admin

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Protected: 3D optics in thicker sections

Posted on February 27, 2012 by admin

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Sunday 02-26-2012

Posted on February 26, 2012 by admin

9:00 A — 11:10 P Etching 4H sample. Testing 4H sample on STORM. 4H has very weak switching.  Might be new Glox not working.  Or extra resin?  see STORM post. Confocal of new in situs.  en-Pc H3-750 failed.  en still … Continue reading →

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STORM 02-26-12

Posted on February 26, 2012 by admin

Laser Powers: 750: 100 mW 647: 15o mW 561: 150 mW 488: 15o mW sample ‘4H’ (histone H3 stained in all 4 channels as an alignment control). etched sample 22 min.  Looks like all resin is dissolved. very weak switching. … Continue reading →

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Saturday 02-25-12

Posted on February 25, 2012 by admin

10:45 A — 6:00P, 8:00P — 12:45 A in situs day 3 working on 3D data analysis Calibrated 750 channel from bead images Confocal: image S2 330 nm (looks like intronic en stain and mature mRNA detectable). Confocal: image Psc-hox … Continue reading →

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Friday 02/24/12

Posted on February 24, 2012 by admin

11:00A – 2:20A humidifying sectioning area.  Trimming 4H sample (all for H3 antibodies). 2 hr hot wash of embryos kept strict, followed by PBT rinse, long block (2 hrs+), then primaries 1 hr at RT, then O/N at 4C. taking … Continue reading →

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