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Daily Archives: February 24, 2012
Friday 02/24/12
11:00A – 2:20A humidifying sectioning area. Trimming 4H sample (all for H3 antibodies). 2 hr hot wash of embryos kept strict, followed by PBT rinse, long block (2 hrs+), then primaries 1 hr at RT, then O/N at 4C. taking … Continue reading
PcG bodies
PcG bodies of Pho and Psc.
STORM 2-24-12
Laser powers 750: fix to 100 mW 647: 175 mW 561 (at 200 mW activation) fix to 150 mW 488: 200 mW 405: 1.5 mW Imaged S6 500 nm, single section with hox expression dot. Arraying sample of S7, engrailed … Continue reading