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Monthly Archives: January 2012
Tuesday 01/31/12
10:00 A — 12:55 A Flip fly cages (MP08 and yw) Review JCC app. Submit JCC app. Working on snail paper final results/model section. Syncing datafiles on snail patterning sectioning: Cut 2 slides of s04(13-introns), one of slide 1, one of … Continue reading
Monday 01/30/12
10:00 A – 8:00 P Projecting snail data (MP08_20x) Fixing minor issues in JCC app from N. Francis suggestions uploading and formatting JCC app should have submitted university letter sooner, Matt helps solve. analyzing MP08 rescue data. 20x images not … Continue reading
Zhuang Lab VPN from linux
Install Remmina Remote Desktop Client (also available through Ubuntu Software Central). Download Harvard’s Cisco Client (.sh file) from: https://core-vpn-1-gw.fas.harvard.edu/CACHE/stc/4/binaries/vpnsetup.sh Alternatively follow the connect to vpn link from the Harvard vpn site. This tries to launch a web/java version of Cisco. If that fails to … Continue reading
Sunday 01/29/12
10:30 A – 2P, 4P – 12:30A working on snail simulations finally found bug in simulation: conn_map missused – entries are width of connections, not indices of cells. configured remote connection on Linux laptop to vpn onto Monet (see post … Continue reading
Saturday 01/28/12
11:00 A confocal O/N scans still running (22/26 embryos, started at 10 P, 35 sections, speed 5). Extended run time until 3P. Literature search for good Drosophila Cohesin antibodies wrote to Dale Dorsett, might have Rad21 antibody to send, recontact … Continue reading
Protected: progress report
There is no excerpt because this is a protected post.
Friday 01/27/12
10:00A check 750 dye-efficiencies using STORM 2 lasers. Detailed observations recorded on Embryo_staining database. multi-histone label sample 2 looked alright by conv imaging on STORM, even 488-ftz is detectable. New microtome installed in lab, training 12-2P. Oven did not reset … Continue reading
Thursday 01/26/12
10:00 A – 7:15 P revise JCC proposal, send to Carl and DB. resin embedding of direct labeled multi-histone modification tagged embryos Finished writing new imclean GUI, orients, trims, and recolors embryos. Updated git repo. updated JCC figure, sent … Continue reading
Wednesday 01/25/12
10:20 A Analyzing confocal images from testing antibodies WGA-488, H3K27me3-Cy3B (red), H3K4me3-Cy5 (blue) running LSM files through imviewer install TeXMaker on desktop working on fellowship applications BIRS proposal for 2013 5 day workshop rejected. Contacted co-organizers to re-stratagize. Writing imviewer … Continue reading
Tuesday 01/24/12
10:00A – 1:00 A Finish v5 of JCC draft, sent to CB and DB. New version of figure for JCC app. 2 new sna[D] bottles for virgin collection to remake MP12 rescue line. DB MP12 flies flipped back to vial … Continue reading
Posted in Summaries
Tagged communication, confocal, fly work, staining
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