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Daily Archives: January 14, 2012
STORM imaging progress update
Review of recent progress on multicolor STORM imaging of gene regulation. WGA labeling membrane in 100 nm section. little Hb detected (red stain mostly background). en-dig stains well with H3K27. just need to cut it better. AbdA (red) and Ubx (green) … Continue reading
Saturday 01-14-12
11:00A – 7:50 P No engrailed stain No evidence that short fix, antibody label, then in situ, actually labels mRNA efficiently. No hb-mRNA detected. attempted imaging of en-dig (cut yesterday) 50 min etching removed all cells, only bits of yolk … Continue reading