Monthly Archives: December 2011

Monday 12/26/11

Posted on December 26, 2011 by admin

Working on figures for snail dynamics paper: wt vs MP08 comparison sent draft of figures and captions to Jacques (earlier version of wt vs MP08 sent with updated dynamics figure to ML yesterday). sent recommended reading to Katherine.

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Sunday 12/25/11

Posted on December 25, 2011 by admin

writing new script for analyzing batches of snail data.  Using each slide as separate case called through the slides and switch command. fit sigmoids to right half of data compute coefficient of variation and mean expression for ‘on’ cells, defined … Continue reading →

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Protected: minus snail distal enhancer update

Posted on December 22, 2011 by admin

There is no excerpt because this is a protected post.

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Wednesday 12-21-11

Posted on December 21, 2011 by admin

7:40 A – 11:00P breakdown O/N confocal of MP08 write script to extract nuclei from cytoplasmic localized mRNAs (sog and snail).  Extract nuclei for s05_MP08_b. first pass dot counting for s05_MP08_b and for snaD_b.  Data not analyzed yet. cutting samples … Continue reading →

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Tuesday 12/20/11

Posted on December 20, 2011 by admin

10:50 A – 8:30P remove engrailed and hox stained and embedded embryos from 70C oven continue embedding of bcd/ftz/K4 protein/RNA multiplex stains — onto 50:50 resin:EtOH Cut sections: en-flu and AbdA, at both 100 nm and 50 nm.  (7hrs).  good … Continue reading →

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Monday 11/19/11

Posted on December 19, 2011 by admin

10:50 A -7:05 test Invitrogen DNA dyes in whole-mount yw 2-4 hr embryos. embedding engrailed and hox labeled embryos, in 70% resin: 11:30A washing out WGA labeling in bcd/ftz/K4 embryos. test Draq5 on sections: also does not label etching new … Continue reading →

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Sunday 11/18/11

Posted on December 18, 2011 by admin

10:40 A -8:40 P Troubleshooting sodium ethoxide etching: 2 washes of 30 min each gives good etching, 100 nanometer sections of embryos are still visible, but resin is gone.  Resin otherwise still visible by confocal.  not readily detected on STORM2. … Continue reading →

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Saturday 12-17-11

Posted on December 17, 2011 by admin

12:00P – 1:00 P, 8:45P – 10:20 P O/N confocal only finished 12 embryos between 8p and noon, extended run to finish remaining 4 selected on slide. mount all MP08.  mount subsets of embryos: eng-dig, eng-fl, antp+ubx, AbdA+ubx. remaining embryos … Continue reading →

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Friday 12-16-11

Posted on December 16, 2011 by admin

10:00A – 6:00P, 8:00P – 9:30P successful 3D pan-histone nuclear reconstructions bcd, ftz, H3 in situs post primary reaction, day 2.  incubating in primary antibody 2nd round with in situ primaries added to solution O/N at 4C. new in situs … Continue reading →

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Thursday 12-15-11

Posted on December 15, 2011 by admin

7:45 A – 8:30 P stopped O/N confocal run, only finished 6 embryos (11P recording start until 7:45A stop). hot washes for in situs (engrailed, ubx, antp, and MP08) split bcd, ftz, H3K4 embryos, start in situs with half of … Continue reading →

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