10:00A – 6:00P, 8:00P – 9:30P
- successful 3D pan-histone nuclear reconstructions
- bcd, ftz, H3 in situs post primary reaction, day 2. incubating in primary antibody 2nd round with in situ primaries added to solution O/N at 4C.
- new in situs day 3. Not mounted or post-fixed, washing O/N at 4C in PBT
- set up confocal O/N 16 embryos snaD hets