12:00P – 1:00 P, 8:45P – 10:20 P
- O/N confocal only finished 12 embryos between 8p and noon, extended run to finish remaining 4 selected on slide.
- mount all MP08. mount subsets of embryos: eng-dig, eng-fl, antp+ubx, AbdA+ubx. remaining embryos rinsing in PBT 1P – 8:45P.
- Rinse out primary antibody rpt on in situs for bcd, ftz, and H3K4 stained embryos. Rinsing in PBT 1P – 8:45P
- Set up new confocal 12 MP08 embryos
- en-in situs now post-fixing in GA, 45 min.
- bcd, ftz, H3K4 second labeling round, blocking 9:20 – 10:00P. in secondary antibodies O/N at 4C