Tag Archives: protein

Saturday 12-17-11

12:00P – 1:00 P, 8:45P – 10:20 P O/N confocal only finished 12 embryos between 8p and noon, extended run to finish remaining 4 selected on slide. mount all MP08. ¬†mount subsets of embryos: eng-dig, eng-fl, antp+ubx, AbdA+ubx. remaining embryos … Continue reading

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Friday 12-16-11

10:00A – 6:00P, 8:00P – 9:30P successful 3D pan-histone nuclear reconstructions bcd, ftz, H3 in situs post primary reaction, day 2. ¬†incubating in primary antibody 2nd round with in situ primaries added to solution O/N at 4C. new in situs … Continue reading

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Thursday 12-15-11

7:45 A – 8:30 P stopped O/N confocal run, only finished 6 embryos (11P recording start until 7:45A stop). hot washes for in situs (engrailed, ubx, antp, and MP08) split bcd, ftz, H3K4 embryos, start in situs with half of … Continue reading

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