Thursday 12-15-11

7:45 A – 8:30 P

  • stopped O/N confocal run, only finished 6 embryos (11P recording start until 7:45A stop).
  • hot washes for in situs (engrailed, ubx, antp, and MP08)
  • split bcd, ftz, H3K4 embryos, start in situs with half of the vials, post-fix and prep other half for embedding.
  • Insight analysis (setup by hand) for all of the H3 data
  • sectioned MP09 embryos, 6 slides worth.  use to test out DNA dyes for labeling after sodium ethoxide etching.
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