Monthly Archives: November 2011

Wednesday 11/30/11

Posted on November 30, 2011 by admin

10:30 – 1:30A checking in situ stains: bio and WGA stains don’t seem to have worked?  (ubx-bio, Antp-bio, and en-bio slides all no stain) should have put shp in 555 as well as 750 to confirm stain. 🙁 hb finally … Continue reading →

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chromatin regulation

Posted on November 30, 2011 by admin

Selected ubiquitous genes flanking Antp complex: Taf1 (left) and alphaTub84B (right).  Need to clone and make probes for these at some point. Look at maintenance of gene activation –how to keep a gene on. Does it require enhancer looping.  How … Continue reading →

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Protected: Analyzing new cc13 data

Posted on November 30, 2011 by admin

There is no excerpt because this is a protected post.

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Tuesday 11-29-11

Posted on November 29, 2011 by admin

9:30A -7P, 8P-11:45A STORM imaging of H3K4me3, 34 positions Rec letters for Madhurima Rinse out secondaries on antibody tests, in Hoechst, 1:30P rinse out primaries on new in situs, in block, 1:30P rinse out primaries on bcd,hb stain, in block, … Continue reading →

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Monday 11-28-11

Posted on November 28, 2011 by admin

10:40 A – 12:30 A PBT rinse for embryos, move to block stored block is cloudy at top again. Make new block with filter sterlized PBS, add new tween 20 separately (150 uL in 15 mL). Bike to MGH to … Continue reading →

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testing new antibodies

Posted on November 28, 2011 by admin

New antibodies to test from Bernstein lab / Manching EED. Mouse millipore 17-663.  .7u/mSUZ12. NG1791537 SUZ12 mouse millipore 17-661 .7. NG1806860 Bmi1 mouse invitrogen 37-5400. .5. 786601A EZH2 39901. Active motif rabbit. 27210001 Mouse anti-H3K27me3.  Ab6002. GR50184-2 rabbit anti-H3K27ac active … Continue reading →

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Protected: validation of distal repression

Posted on November 28, 2011 by admin

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Sunday 11-27-11

Posted on November 27, 2011 by admin

5:30P – 8:30P Washing out in situ probes Need to check filters before starting hybe reaction to see that embryos aren’t stuck to top filter. Can remove filter and aspirate off probe to reuse from bottom sample. Flip fly cages … Continue reading →

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Friday 11-25-11

Posted on November 26, 2011 by admin

Analyzing snail rescue data — need to save visual validation of dot counting regions. A good number of the new runs undercount dots, try to fix next week.

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Saturday 11-26-11

Posted on November 26, 2011 by admin

4:30 P – 6:15P New wt collection cages (2 small) in situs (O/N collection, prepped previously) Ubx-dig, Antp-bio (blue 1) AbdA dig, Ubx-bio (blue 2)  E(Pc)-dig, tou-dig, en-bio (clear 3) added probes, shaking at 1200rpm at 55C. 6p.

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