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Daily Archives: January 20, 2012
Friday 01/20/12
10:10 A -11:00 P labeling primary anti-histone antibodies (see Alistair_STORM_dyes log): pan-H3, H3K4me3, H3K27me3, and H3K27ac. Need to filter Ac and K27 through column before binding. Also filtered H3 and K4 — dilutes sample sufficiently to allow for multiple subsequent … Continue reading
3D histone views
3D reassembly of microsection stack of STORM images of individual nuclei with pan-histone marker. Some alignment issues still, but worth a comparison