Daily Archives: February 10, 2012

Friday 02/09/12

10:10 A — 11:20 P move samples from 4C, change 100% EtOH STORM: setup 10x objective for finding samples. substantial offset, need to calibrate. Need to set laser powers to zero but have lasers on before starting dave! Ordered Bloomington … Continue reading

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whole-mount tests 02/09-10/12

slide H1: 4 color anti-nuclear lamin test   slide H2: Directly labeled antibodies: Dm01-488(green) H3K27me3-Cy3B (red), H3-Cy5 (blue), H3K27ac-750 (not shown) slide H4: H3K27me3-Cy3B (red) and H3K4me3-Cy5 (blue) along with 405 background (green). slide H4: earlier embryo: H3K27me3 ‘clusters?’  (red) … Continue reading

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