11:00A – 8:00P,
Coding
- Accelerating integrated 4 color chromatic, drift correction into code to convert molecule list to corrected tif images.
Notes from STORM presentation
- STORM2 power report,
- At 40 x 40 um at 60 Hz:
- 20 mW 750 at 150 mW
- 40 mW 647 at 150 mW
Multiactivator:
- alternating activating lasers in bursts. Leave activator off while bleaching with 647.
- Frames need to match camera
- Comments TIRF/Epifluorescence long discussion of epiluorescence.
- Does the objective choice affect TIRF? Is this more a light sheet than a TIRF. the beam itself is hitting the sample, not just the effervescent beam.