2:00P — 8:30P
- Calibrating new bead imaging: 1:20 then 1:500 540 beads + 1:500 Q-dots-705. Too dense.
- Dilute a further 1:500. Q dots good density. Blinky, excited best by 405 or 488. Look good in 647, not really visible in 750 channel — more visible in 561 than 750. 🙁
- Hard to unmix the 488 excited Q-dots-705 from the 540 beads (potentially feasible by intensity).
- 3D bead measurement not mapping IR 647 and Vis 647 to the same plane.
- mites detected in fly room! Evacuated fly stocks to Natio for time being. Keep under observation!
- STORM imaging of Antp locus and H3-750 with weak signal from H3K27-647.