Saturday 06/30/12

Posted on June 30, 2012 by admin
  • Flip fly cages.  Old Pc-GFP dying –> freeze.
  • Need to characterize loss of snail mRNA in snail nulls better.  ~early cc14 levels look like even half peak cc13 + degradation: ~85 transcripts per cell (highs just over 100).  late cc14 20-30 transcripts transcripts per cell.  Expect to drop to 10% (~10 transcripts/cell) in 30 min (close to background levels 5-15 range per nucleus, depending on stain quality).  Measure embryos still in the 20-30 at cephalic furrow.    Should account for a 10 – 20% transcription rate judging by presence of nascent transcripts.   With 10% cells transcribing expect about 22 mRNA/cell after 30 min. 
  • working on z-color alignment.  xy-alignment by nuclear lamina looks good.  750 and 647 beads getting mapped 1000 nm apart.  Even when using the same calibration curve and by eye the distribution of x elongated and y elongated is equal.  All mapped positions are also outside of range of sample.  Maybe its the drift correction that’s miles off.  
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