Monthly Archives: August 2012

Friday 08/31/12

Posted on August 31, 2012 by admin

9:30 A – 7:30P, 10:30 – 12:50A Break down O/N mp10a run. save mp07 data.  All look good, 7 images finished completely, last image starts at the top of the mRNA field. etching S1 AbdA, AbdB, Ubx, K27me3. Issues booting … Continue reading →

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Protected: snail bistable analysis

Posted on August 30, 2012 by admin

There is no excerpt because this is a protected post.

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Thursday 08/30/12

Posted on August 30, 2012 by admin

10:15 A – 12:00A Admin stuff: Nstar application faxed.  HHMI benefits sent to old address, can get canceled and resent after 30 days. break down STORM4 run. Organizing snail data [spreadsheet 0AjSqkxgziU1YdGxMQnMtd0ZXaDhDY09BbHY2SmZqX2c sheet=1 602 602] Fixed bug and got sample code … Continue reading →

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Wednesday 08/28/12

Posted on August 29, 2012 by admin

9:50 A-7:00P, 8:30P – 11:50P pick up drive from HCBI STORM4 lost focus O/N.  Reset focus, continue imaging. Trying to get good matches for pnrPr dynamics in simulation, not just end states. Dyes not switching well in 15 min etched … Continue reading →

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Tuesday 08/28/12

Posted on August 28, 2012 by admin

9:30 A – 7:00PM, 7:45P – 12:00A 20 min etch Pc-647 sample.  Turnkey inspection looks over-etched, most embryos quite sparse. Forgot that previous MP07 samples have Draq5 not DAPI.   Extracting nuclear masks manually through inverting DAPI channel and using … Continue reading →

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STORM4 alignment

Posted on August 27, 2012 by admin

Adding cylindrical mirror into beam path on STORM 4 and checking camera alignment and beam alignment Camera alignment Unscrew set-screw attaching camera port to microsocope body.  The hex rench mounted on the back of the microscope fits this set screw. … Continue reading →

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Sunday 08/26/12

Posted on August 26, 2012 by admin

3:30 PM -:715P, 8:30P – 10:30P STORM run lost focus.  Haven’t analyzed yet at which position / how many movies lost. Setup new collection of positions.  All pretty old embryos in this batch, K27me3-647 stain doesn’t look great, channel has … Continue reading →

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Reflections on Petruk et al Cell 2012

Posted on August 26, 2012 by admin

TrxG and PcG Proteins but Not Methylated Histones Remain Associated with DNA through Replication, Cell (2012), Result 1 (Fig 1):  ‘K27’ ‘K4’ don’t associate with PCNA labeled nuclei.   comment: At gastrulation K27 and K4 levels are much much lower than … Continue reading →

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Saturday 08/25/12

Posted on August 25, 2012 by admin

10:30 A – 7:30P, 8:3oP – 10:40P check O/N confocal run, 13 embryos 2 channels 70 sections on speed 5 started at 8P, 12/13 finished. (several embryos were not substantially flattened, hence the extra sections). expansion of lens has changed … Continue reading →

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Friday 08/24/12

Posted on August 24, 2012 by admin

10:15 A – 9:30P running analysis on images sent by Mounia.   see post4160. Write up snail analysis updates (see post). Trying to fix lsm format error on image 13 and later of m106a.  Unable to ID parse error. Write … Continue reading →

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