Monthly Archives: July 2013

Chromatin Experiments To Do

Posted on July 11, 2013 by admin

in situs BX-C Expression and DNA locus multiplex whole-mount embryo in situs for AbdB, AbdA, and Ubx with dig probes anti-dig-405,647 ultra cryo section embryos. label with WGA-488 on coverlgass, then STORM the RNA labels. DNA-FISH BX-C or individual AbdB, … Continue reading →

Posted in Research Planning | Comments Off on Chromatin Experiments To Do

Thursday 07/11/13

Posted on July 11, 2013 by admin

10:00 A – 7:00P, 8:30P-10:00P STORM Finish O/N run of PhM-flag-488, Ph-647 take bead images transfer data to ProRAID Set up analysis to run on Cajal Embryo in situs wash out probes check staining: All embryos washed off! 1 tiny … Continue reading →

Posted in Summaries | Tagged , , | Comments Off on Thursday 07/11/13

Wednesday 07/10/13

Posted on July 10, 2013 by admin

10:00 A – 7:40 P, 8:30 – 10:00 P General laboratory safety training refresher check HoxD server jobs: still heavily backed up check STORM analysis / drift correction: Y complete. Others still running. update notebook entries from yesterday. Review working … Continue reading →

Posted in Summaries | Tagged , , | Comments Off on Wednesday 07/10/13

Protected: project 2 07/10/13

Posted on July 10, 2013 by admin

There is no excerpt because this is a protected post.

Posted in Genomics | Comments Off on Protected: project 2 07/10/13

Tuesday 07/09/13

Posted on July 9, 2013 by admin

9:30 A – 7:00 P, 8:15 P – 11:00 P chromatin got 10 kb segments to run. Still lots of crashes, some substantial regions lack probes if we’re fragmenting sections so we have to stitch backtogether, might as well do … Continue reading →

Posted in Summaries | Tagged , | Comments Off on Tuesday 07/09/13

Monday 07/08/13

Posted on July 8, 2013 by admin

9:30 A – 7:00 P Lab meeting Presentation Notes Journal Club Notes Quick Project2 group discussion. Uploaded presentation stub for Tuesday Ph project quick look at recent STORM data performing chromatic bead analysis to align color channels. Psc PhM storm2 … Continue reading →

Posted in Summaries | Comments Off on Monday 07/08/13

Protected: Ph Data Pc Psc effects

Posted on July 8, 2013 by admin

There is no excerpt because this is a protected post.

Posted in Chromatin | Tagged | Comments Off on Protected: Ph Data Pc Psc effects

journal club 07/08/13

Posted on July 8, 2013 by admin

Steven presenting CRISPR cell paper One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering Background CRIPR repeat sequences separated by spacers. Spacers are different, match different bacterial phage sequences. components: Cas1, Cas2, Csn2 (involved in encorporating … Continue reading →

Posted in Journal Club | Comments Off on journal club 07/08/13

Protected: lab meeting 07/08/13

Posted on July 8, 2013 by admin

There is no excerpt because this is a protected post.

Posted in Lab Meeting | Comments Off on Protected: lab meeting 07/08/13

Saturday 07/06/13

Posted on July 6, 2013 by admin

Working remotely from Wareham. chromatin project HoxD project BLASTed hoxD probe sets against Drosophila genome. Haven’t even downloaded mouse genome Download and build BLAST+ and LegacyBLAST libraries for mouse genome. Screen primers against mouse genome some loci still refuse to … Continue reading →

Posted in Summaries | Comments Off on Saturday 07/06/13