Monthly Archives: July 2014

Wednesday 07/23/14

10:00 am – 12:15 am Chromatin Project Sequential staining snapped coverslip tried mounting half on flow-system anyway this leaks — not enough pressure seal in the gaskets (especially relative to pump pressure of manual syringe) try manual hybes of second … Continue reading

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Protected: Highlights for lunch meeting

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Tuesday 07/22/14

11:25 am — 11:45 pm (biking Minuteman path, 8:15a-11:25a) Goals Ajaz’s sample project 2 data analysis chromatin data analysis Project 2 analysis see protected post Chromatin Project Data analysis fitting E06 and G2toG4 data trying to analyze BX-C data from … Continue reading

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Protected: project 2: 07/22/14

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Monday 07/21/14

9:30 am – 5:00 pm, 9:30 pm – 11:45 pm Lab meeting 10:00am – 1:30pm Steven presenting, see notes Project 2 analysis see protected post STORM imaging L3 E07toE09

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Protected: project 2 update

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Protected: lab meeting 07/21/14

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Protected: project 2: E2

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Wednesday 07/16/14

10:00 am – 12:00 am Sequential Staining all at 37C (heated objective) flow 2X SSCT to rinse out storm buffer prehybe in 2x SSCT + 50% formamide, 15 min hybe 30 min with S1toe5 (3uL in 1 mL hybe dilution … Continue reading

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Protected: project 2: control

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