Monthly Archives: April 2015

Thursday 04/30/15

9:00 am – 7:30 pm, 9:00 pm – 11:30 pm Thesis defense finalizing hat for Hao 9am-10am Hao’s Thesis defense 10am-11:15 am more finalizing hat 11:15-11:45 Discussions 11:45-1:00pm discussion of AO with HB and BB discussion of BB’s sequential stain … Continue reading

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Protected: Hao practice dissertation

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Friday 04/24/15

9:00 am – 5:00 pm, 7:45 pm – 10:45 pm practice talk (9am-10am) Lab meeting 10am – 1:30pm see protected notes Embryo MERFISH project Embryo staining STORM3 observations of cut sections some individual spots look promising. despite high concentration of … Continue reading

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Thursday 04/23/15

9:00 am – 10:15 pm Microscope booking booked STORM3 for imaging this evening STORM2 in use, Andor camera moved to STORM5 construction scope STORM4 may be available for checking samples re-applied for expense code for confocals. registered for 7pm – … Continue reading

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Wednesday 05/23/15

9:00 am – 5:30 pm, 9:00 pm – 10:00 pm Morning tasks Finish ultra-cryo cleanup (put knives away) Discuss drive issue with Dell. system currently configured with only 4 bays active need RAID controller to connect 8 bays together and … Continue reading

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Tuesday 05/21/15

9:00 am – 10:50 pm Computer maintenance finish install of updates. Shut-down Morgan install power adapter for USB 3.0 cards bridge to DVD drives investigate motherboard for ID# (Dell special, no additional info) and RAID card (none evident) restart computer … Continue reading

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Monday 05/20/15

10:00 am – 5:00 pm Meetings discussion with NL about MERFISH 10 am – 11 am reply to E Furlong about MERFISH BWF prep call foundation about slide submission rehearse presentation timing feedback from Jeff on slides feedback from Bogdan … Continue reading

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Sunday 05/19/15

5:00 pm – 12:00 am MERFISH Transcriptome building saving additional FPKM data per cell line, per chosen isoform, rebuilding FASTA file with short gene names. running with maxFPKMperType > 1E-5 & maxFPKMperType < 50E3 and fasta length over 2000 bp … Continue reading

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Friday 05/17/15

9:00 am – 5:00 pm 9:00 am – 3:00 pm review and prep slides for group meeting journal club lab meeting (see notes) 10am – 1pm reformatting figures for Ph paper Transcriptome design might have been faster to use cufflinks … Continue reading

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Protected: lab meeting: 04/17/15

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