Monthly Archives: June 2015

9:45 am – 7:30 pm, 8:30 pm – 10:00 pm RNAi setup RNase digestions fixed cells (probably too late for these guys, actually started Tuesday night). Primer design would be useful to do by batch in matlab rather than manually … Continue reading →

9:45 am – 5:15 pm Testing RNAi knockdown RNA isolation (assuming you cultured in 6 well plates – scale accordingly) * Wash cells in cold PBS (with Kc, might have to resuspend, spin down, wash in PBS, then spin down … Continue reading →

9:50 am – 8:00 pm presentation prep tuning talk and slides Coding rewriting Chromatin Cropper New Organization 100% of the action of the GUI should be executable as command line functions (currently the main processing “steps” are still steps of … Continue reading →

9:40 am – 11:00 pm Heat shock experiments continue cell prep LN2 treatment, 5 min 1M HCl bath RNase A digestion (30 min at 37C) SSCT wash de-ionizing fresh formamide prepared new formamide buffers prehybe and store at 4C Bogdan … Continue reading →

10:00 am – 5:15 pm 10:45 pm – 11:30 pm Tasks discussed with Dell again about memory (they make this unnecessarily complicated). Experimental goals for today check progress RNAi knockdowns purify more RNA for RNAi set up new RNAi knockdown … Continue reading →

9:50 pm – 11:45 pm RNAi RNA prep set up T7 reactions 10 x 9 = 90 uL NTP buffer mix 2 x 9 = 18 uL T7 pol mix 2 x 9 = 18 uL RNasin 6 x 9 … Continue reading →