Monthly Archives: July 2015

Friday 07/31/15

Posted on July 31, 2015 by admin

9:00 am – 5:00 pm Lab meeting see notes Discussion talk with Ting talk with Xiaowei chromatin project project update meeting see google-docs slides Ph Project need to downsample localizations (2-fold?) an re-compute statistics and show similar distribution.

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Thur 07/30/15

Posted on July 31, 2015 by admin

11:00 am – 7:30 pm, 9:30 pm – 10:45 pm (9:00 am – 11:00 am volleyball practice) Chromatin Cell plated Kc167 cells (in SFX) on glass my more recent flask with the short Trypsin treatment is doing well. the original … Continue reading →

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Protected: lab meeting: 07/31/15

Posted on July 31, 2015 by admin

There is no excerpt because this is a protected post.

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Wed 07/29/15

Posted on July 29, 2015 by admin

9:10 am – 8:00 pm, 9:30 pm – 12:00 am To do confocal imaging design primers re-read and submit review Done confocal imaging (no staining) submitted review (done) feedback for Hao on essay (sent) Embryo staining no signal with 37C … Continue reading →

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Protected: Ph-Polymerization colocalization control

Posted on July 29, 2015 by admin

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troubleshooting hb stains

Posted on July 29, 2015 by admin

Purpose to start MERFISH experiments in Drosophila embryos Plan: use Biosearch probes to optimize RNA labeling using DNA probes currently using 20mers directly labeled. These aren’t working. Images from Shawn Little’s paper (Gregor lab) My RNA hb stains zoom out … Continue reading →

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Tuesday 07/28/15

Posted on July 28, 2015 by admin

9:30 am – 7:30 pm, 9:30 pm – 11:45 pm Ph polymerization reviews back writing reply goal to respond this week to do Ph-Flag / Ph co-localization data DNA FISH long range contact frequency (hold off) Live imaging of chromatin … Continue reading →

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Monday 07/27/15

Posted on July 27, 2015 by admin

9:20 am – 10:00 pm Reading I’m looking for new insights to improve our genome wide prediction of PREs Schuttengruber et al Cell Reports 2014 on PRE evolution Zheng et al 2012 develop computational predictor this uses ChIP data to … Continue reading →

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Sunday 07/26/15

Posted on July 26, 2015 by admin

3:30 pm – 6:30 pm embryo stains rinse embryos in pre-hybe/ was buffer, 1 hr rinse embryos in PBT stain a subset with WGA mount this subset for confocal imaging in Prolong Diamond Chromatin project discussion on project progress with … Continue reading →

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Friday 07/24/15

Posted on July 24, 2015 by admin

9:15 am – 7:15 pm PRE mapping Ringrose 2003 predicted PREs http://www.techfak.uni-bielefeld.de/~marc/pre/hit_list.html Ringrose 2003 is dm1. Need to lift over to dm3 before we can map. lift over, plotted correlation. serious computer crash (windows froze while unpacking .gz file), matlab … Continue reading →

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