Tuesday 07/28/15

9:30 am – 7:30 pm, 9:30 pm – 11:45 pm

Ph polymerization

  • reviews back
  • writing reply
  • goal to respond this week

to do

  • Ph-Flag / Ph co-localization data
  • DNA FISH long range contact frequency (hold off)

Live imaging of chromatin

  • dCAS9 for Drosophila sequence
  • transfection vector for Drosophila

Reagents

  • dCAS9-fused to Fok1 (need to add mmaple3) from addgene (here)
  • repeat sequences:
    • the “dodeca repeat” ACGGGACCAGTACGG
    • the “359 repeat” GGGATCGTTAGCACTGGTAATTAGCTGC,
    • the “AACAC” repeat: AACACAACACAACACAACACAACACAACACAACAC
    • the AATAT repeat: AATATAATATAATATAATATAATATAATAT
      DrosophilaRptLocations
This entry was posted in Summaries. Bookmark the permalink.