Monthly Archives: August 2015

Sunday 08/30/15

11ish – 4:30 pm, 8:00 pm – 11:00 pm remotely 5:30-8:00 Analyzing Hi-C data effect of flank size on isolation score: scaling exponents within a domain have a troubling dependence on domain length. drafting description of Hi-C comparison see Revision … Continue reading

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Monday 08/31/15

9:00 am – 10:40 pm Live imaging live imaging of telomere-mmaple3 construct on STORM2 need to correct live drift so we’re stuck with auto-correlation for these, no need to acquire beads blinking pretty well in current settings 800 mW set … Continue reading

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Protected: Lab Meeting, 08/28/15

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Thursday 08/27/15

9:30 am – 7:00 pm, Lab meeting prep working on slides for Group B presentation, 9:30 am – 1:30 pm Two-color data analysis interesting results from ANT-C G6 quantification median overlap ~25%, median entanglement ~0.06 need to make figures still … Continue reading

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Wednesday 08/26/19

9:30 am – 9:00 pm Goals ChromatinCropper2 two color data for ANT-C G6 Spot finding for two color BX-C F6 data ChromatinCropper2 two color data tape station of sequencing prepped library for Ph-KD and control qPCR of sequencing prepped library … Continue reading

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Tuesday 08/25/15

9:30 am – 10:15 pm STORM finish overnight run switching still looks decent setup data to analyze on MORGAN. updated z-calibrations for both 647 and 750 parameters fitting bead data for drift correction all jobs launched before 1pm (probably need … Continue reading

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Monday 08/24/15

9:30 am – 5:15 pm, 8:20 pm – 11:30 pm Ph-polymerization Manuscript to do STORM section, reply to notes in main text Finish Layout Supp figure 2 and Supp figure 4 added supplement captions form both Check the legend to … Continue reading

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Friday 08/21/15

9:30 am – 7:00 pm Ph project rereading and revising manuscript sent comments back to team revising layout of Fig 5. revising response to reviewers sent updates back to team STORM imaging Jeff realigned lasers on STORM4 table, fixing the … Continue reading

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Thursday 08/20/15

9:30 am – 1:30 am Microscope troubleshooting might be able to try 2-color imaging in STORM5 with George E and shutters. doesn’t work. No emission filter to block 405 2 color conventional images of my stains look great though. trying … Continue reading

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Wednesday 08/19/15

9:00 am – 11:30 pm Visiting postdoc candidate interviews presentation 11 – 1:30 pm lunch 1:30 – 2:00 pm individual meeting 4-4:30 pm STORM finish imaging 2 color take bead images for z-calibration take bead images for chromatic correction Chromatin … Continue reading

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