9:30 am – 7:00 pm,
Lab meeting prep
- working on slides for Group B presentation, 9:30 am – 1:30 pm
Two-color data analysis
- interesting results from ANT-C G6 quantification
- median overlap ~25%, median entanglement ~0.06
- need to make figures still
Meetings
- requested to make MERFISH slides for grant meeting
- practice talk to be given Sept 4 (next Friday)
Sequencing prep
- ran tape-station on 1:100 dilution samples
- looks good, library length peaks are 297, 302 and 307.
- Problem except I cant’ connect to the data folder: https://data.rc.fas.harvard.edu to get the raw data to post here.
- submitted request to RC help for troubleshooting.
- computing final concentration (now that we have average library length).
- 600 nM * (450/300) = 900 nM starting. Desired 10 nM. So my 1:100 dilutions are around 9 nM, pretty good.
- submitted sequencing request (still did not submit physical samples, should follow up with Christian).
To do
- reads per domain for all domains (by color) compared to green domains
- analyze the BX-C F6 data
- images of the BX-C F6 data for presentation
- quantification comparison of BX-C F6
For later
- restain Ph with shorter fix time
- try Ph stain on MeOH fixed cells