9:00 am – 11:30 pm
Visiting postdoc candidate interviews
- presentation 11 – 1:30 pm
- lunch 1:30 – 2:00 pm
- individual meeting 4-4:30 pm
STORM
- finish imaging 2 color
- take bead images for z-calibration
- take bead images for chromatic correction
Chromatin analysis
- selecting images of internal domains for presentation for XZ
- Analyzing SIM data. Images look on par with confocal images. Registration looks similar. Cell numbers are lower.
- processing the 2-color data for BXC and L2 in Ph-KD
- updating ChromatinCropper2 GUI to handle multicolor
- Analyzing 2-color data
- almost all the 750 spots disappeared in both conventional and STORM images.
- I swear these were visible earlier.
- I think maybe its a focal plane issue, somehow the z-offsets are larger now.
- Maybe Hal’ interpretation of the direction of the offset has also changed, +/- relative to wavelength is interpreted differently on the different systems.
Presentations
- starting slides for meeting with XZ tomorrow
- touch up slides for discussion with post-doc candidate
- not so many cartoons of chromatin, here words will actually be better.