9:30 am – 1:30 am
Microscope troubleshooting
- might be able to try 2-color imaging in STORM5 with George E and shutters.
- doesn’t work. No emission filter to block 405
- 2 color conventional images of my stains look great though.
- trying to image on STORM4
- issues with quadview – 561 channel not working
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Writing
- revise draft of Ph-manuscript and send replies to Nicole and Ajaz
RNAi experiments
- Day 4 for KD. Lyse cells, perform RNA extraction.
- collected 7 tubes of cells, concentrated in spins
- just froze cells in lyses buffer at -80C. Will continue these when I have time.
- fix some cells for imaging backup
- prep cover slips
- plate cells, allow 3 hours to attach
- fixed 4 coverslips of Ph KD and 4 coverslips of mock.
- these may be necessary for continuing the multicolor experiments
Meetings
- discussion with XZ about grant, 11am
- update on revision experiments, 1pm
Live imaging
- TIRF2-mmaple on STORM4
- sample don’t look promising
- snapped some images, saved on STORM4, need to copy over for update meeting
- TIRF2-GFP on STORM3 (Bogdan, 6pm)
- samples not well adhered (polylysine treatment was omitted).