Monday 08/11/14

9:30 am – 5:10 pm, 10:00 pm – 12:30 am

Meetings

  • group meeting (notes)
  • journal club (notes)

Project 2

STORM

  • finish staining chr-L4 A01, A02, A01 to A03.
  • setting up to image A01 to A03
  • make new Glox solution
  • staining failed. Check probes by Gel. I guess plate-columns don’t work so well…

Coding

  • modified ChromatinCropper to have option to use conventional images taken immideately prior to storm data to drift correct the conventional images in multi-color taken at the very beginning of the run.
  • tested function and incorporated into main GUI
  • currently on CCupdate branch. Ready to merge back into master.

Probe diagnostics

  • Gel order: gel 1: 1,2, 9,10, 17,18, P3:1,2, gel 2: F1-F7, E9-E6
  • these actually look reasonable,
  • though maybe the concentration of the L4 probes is extremely low since these samples are taken from purified final probe (15 uL) and the L2 and L3 samples are equally bright but taken from diluted RT reactions (~800 uL). Should nanodrop.

L4L3L2_probes_labeled

Ph Project

  • working on supplement on interpreting cluster sizes and counts
  • explaining STORM vector data
  • explaining geometric distributed data.
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