Wednesday 04/25/15

9:00 am – 6:00 pm

Meetings

  • discussion with patent lawyer
  • discussion with MERFISH team

Computer troubleshooting

  • User Profile Disks might be problem with user session management: http://www.virtualizationadmin.com/articles-tutorials/vdi-articles/general/working-with-user-profile-disks-on-session-based-desktop-deployments.html
  • disabled User Profile Disks (these were not enabled on CAJAL but were enabled on MORGAN when MORGAN started deleting user account data).
  • re-installing local code database on my MORGAN account
  • running OligoArray with 20mers 66C – 76C for all IMR90 transcriptome (37,442 genes)
  • launches about as fast as when running on Cajal, but uses no CPU (maybe saving over the network uses more CPU?)

Tissue section prep

  • Ultratome booked today and tomorrow. I booked Friday after lab meeting

Presentation Prep

  • working on slides for journal-club presentation on past 2 years of Hi-C.

Fly patterning

  • Created Fasta file of all 547 annotated patterning genes (has all the major players), selected longest isoform for each.
  • Playing with embryo section-seq data
    EmbryoSectionFPKM
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