9:00 am – 6:00 pm
Literature
- Gomez-Diaz and Corces 3D genome architecture review
Experiment Planning
Drosophila Patterning screen
- started new Projects folder TF interactions
- Downloaded list of 1000 annotated sequence-specific TFs (GO annotation) from FlyBase. This list has substantial number of non-melanogaster genes to be removed.
- Extract gene IDs, Extract sequences
- Flybase exon download extracts exons of different lengths but mostly overlapping sequence as different exons.
- use transcripts instead, just select longest transcript
Plan
- order tiling probes from BioSearch (30 probes) against two select genes
Potential cover art ideas
- http://eoimages.gsfc.nasa.gov/images/imagerecords/78000/78674/london_lights_2012087_lrg.jpg
Chromatin imaging
- move embryos into 30% sucrose for cutting