Tuesday 03/24/15

9:00 am – 6:00 pm

Literature

  • Gomez-Diaz and Corces 3D genome architecture review

Experiment Planning

Drosophila Patterning screen

  • started new Projects folder TF interactions
  • Downloaded list of 1000 annotated sequence-specific TFs (GO annotation) from FlyBase. This list has substantial number of non-melanogaster genes to be removed.
  • Extract gene IDs, Extract sequences
  • Flybase exon download extracts exons of different lengths but mostly overlapping sequence as different exons.
  • use transcripts instead, just select longest transcript

Plan

  • order tiling probes from BioSearch (30 probes) against two select genes

Potential cover art ideas

  • http://eoimages.gsfc.nasa.gov/images/imagerecords/78000/78674/london_lights_2012087_lrg.jpg

Chromatin imaging

  • move embryos into 30% sucrose for cutting
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