Monday 04/13/15

Posted on April 13, 2015 by admin

9:00 am – 6:20 pm, 8:00 pm- 10:30 pm

MERFISH

  • design new libraries
  • discussed design with team (9:30-10:30am)
  • working on library assembly (8:00pm-10:00pm)

New Libraries (140 gene).

  1. 20mer secondaries 30mer targeting regions 1-16 (shorter than before)
  2. 20mer secondaries 30mer targeting regions 17-32
  3. FAST 20mer secondaries 30mer targeting regions 1-16
  4. FAST 20mer secondaries 30mer targeting regions 1-16
  5. 20mer secondaries 6 on-bits 30mer targeting 1-16. (2 bits per target)
  6. 20mer secondaries 30mer targeting regions 1-16 (4 bits per target)

For later

  1. 20mer secondaries variable targeting

Chromatin tracing

  • Prep for chromatin tracing meeting 10:40 – 2:00pm
  • discussion with Bogdan on strategy 11am-12pm
  • see slides
  • tracing discussion with TW and XZ and team 2:00pm – 6:15 pm

Ph project

  • new draft of figures and text from NF

Abstract Submission

  • submitted abstract for SDB meeting, ~2 hours before deadline (I think). 10:00-10:30pm
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