Sunday 06/14/15

Posted on June 14, 2015 by admin

12:00 pm – 12:45pm, 2:30 pm – 7:00 pm

Animal culture

  • flip fly plate, 12:30 pm
  • passage cells to 75 mm^2 culture flask. Density looks good.

flyMERFISH

  • designing fly transcriptome using Jeff’s new pipeline (as an exploration of new pipeline code).
  • MORGAN updated and rebooted itself.

Notes on using Transcriptome class

  • class annotations for AddAbundances reports only uses cufflinks .fpkm_tracking files. This can’t be true though since the human transcriptome example gets passed my max abundance list from a structure.
  • solved: also says accepts name fpkm pairs. These need to be vectors
  • found minor bug: mode thrown on a double instead of rounded data. Fixing this substantially improves the number of sequences found per gene.
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