10:00 am – 11:00 pm
To do
- PRE correlation analysis
- expression correlation analysis
- design and Green domain probes
- finish updates to ChromatinCropper2
- analyze internal domain data
Correlation analyses
- repeated PRE analysis as discussed:
- recomputed using Cavalli/Tanay labs latest PRE predictions
- examined distance from trend as function of PRE density
- explored PRE density and number vs. volume (compared to length vs. volume)
- could trivially extend to Rg
- loaded expression data, see some weak correlation to explain outliers
- also see (surprisingly) correlation with CTCF peak density to explain outliers
- should combine these two.
STORM analysis
bead calibration
- chromatic calibration still works okay with defaults
- z-calibration: would like to rewrite this function. Less automatic selection of curves and more user input.
Live imaging
- attempt repeat of live imaging
- 2nd round looks good.
- repeated issues with sample movement after pipetting
- really should remember double-stick tape next time.