Saturday 08/08/15

11:00 am – 1:15 am

PcG RNAi

New Knockdowns and cell culture

  • plate Ph, Pc, and mock RNAi cells onto coverglass
    • density looks okay modulo a decent number aren’t sticking
  • isolated cells for RNA extraction
  • fixed plated cells to coverglass for imaging
  • Psc null cells
    • starting to dye for not changing media (despite growing too slowly to be confluent).
    • replaced media on small plate and scraped plate (both these look pretty decent toward confluency).
    • combined media into new small vial, maybe detached cells will attach and settle (took > 24 hrs to attach last time)
    • made new Psc media (using modified Schneider’s from Lonzo and HI FBS).

Imaging BX-C in Ph-KD

  • finish imaging of L2F03F04
  • start imaging of L4E24

Analysis of BX-C internal domains in Ph-KD

  • still fiddling with export options for ChromatinCropper2
  • working on building ChromatinCropper2 GUI. This always takes longer than I think.
    • meanwhile I’m working out what I hope is a better general system for GUIs.

Cell cycle studies

  • prep cells for hybe
  • hybe BX-C to cells.
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