Monday 08/10/15

10:00 am – 5:15 pm, 8:30 pm – 1:15 am

tasks

  • pick up Ph antibody from Patrick at MGH (antibody gift from Jurg Muller)
  • ELYRA training. — with extreme laser power images looked promising. Otherwise images looked substantially less nice than the confocal
  • don’t have compatible version of Zen installed. Applied for download of latest version of Zen Light.

Cell cycle analysis

  • CycA looks promising (for G2).
    CycA_antibody
  • CycB stains (supposed to by cytoplasmic). G2/M marker. Destinction not so good. Stain generally dimmer as well.
    CycB_antibody
  • mPCNA (for s-phase) also looks promising
    mPCNA_stain

Data Analysis

  • processing Ph KD data
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