9:15 am – 6:30 pm
presentations
- revising presentation for Pairing meeting with suggestions from BB (9:30 am – 12:30 pm)
- practice presentation
Experiments
- 1 well of Ph KD and WT took through the RNAi treatment / wash / serum starve with RNA procedure
- 1 well of Ph KD and WT took through RNA isolation
- also isolated RNA from samples which were treated last week.
- samples labeled Ph-WT 1, Ph-WT 2, (not certain on labels here, but QPCR will make clear), Ph-WT 2-day, Ph-Mock 2 day.
- will prep the 4-day samples on Saturday.
- setting up RT reactions to convert to cDNA.
- need to RNase H treat (running low on RNase H). Need to set up QPCR analysis
Literature
- Corces proposes loop extrusion model in review on Guo et al CTCF inversion by CRISPR paper
- also in this issue of Cell, Misteli Hipmap.
- reading Guo et al paper – quite excellent. downloaded supp mat as well, need more time to go through this long paper carefully.