8:30 pm – 11:15 pm
RNA deep-seq prep
- sample order (8 samples): 10-22 n1, 10-22 n2, 2W, 2P, 2-4W, 2-4P, 4W, 4P
- running sample isolation by magnetic polyT beads, following manufacturer’s protocol.
- 3 samples dehydrated in last elution step. I added more elution/fraction buffer to resupsend the dried out beads and reheated to 94C for 1 min.
- this probably incurred some sample loss though. We’ll see in the quantification steps.
- running 1st strand synthesis reaction overnight
cDNA prep for QPCR validation of RNAi
- running RNaseH digestion