Tuesday 10/27/15

9:00 am

Deep seq final prep

  • running tape-station

samplesname, conc pg/uL, index, tube

  • Ph1, 496, 1, A
  • M1, 442, 2, A
  • M2, 665, 4, –
  • 10-22 #1, 652, 1, B
  • 10-22 #2, 516, 2, B
  • 2W, 617, 3, B
  • 2P, 708, 4, B
  • 2-4W, 588, 5, B
  • 2-4P, 686, 6, A
  • 4W, 565, 7, A
  • 4P, 631, 8, A

final

  • A mixed 10 uL of each (as marked above). conc ~2.82 nM, call it 3.0 to be safe
  • B mixed 10 uL of each (as marked above). conc ~3.2 nM, call it 3.5 nM to be safe. 50 uL
  • sent for sequencing.

Interview prep

  • working on slides for phone interview.

Manuscript

  • shrinking figures to fit limitations
  • implemented reference trimming as discussed yesterday.
  • still need BB’s rearranged fig 2 with added legends.
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