Wednesday 06/01/11

9:40 A — 7:10P, 8:00P – 12:15 A

  • Finish ON confocal run of 6x bcd
  • 10A Flip collection cages
  • Working on imviewer_lsm rewrite
  • Interview with David Stern
  • found missing fclose statement in imviewer that fills up memory.  imwrite crashes matlab if target folder is out of space. — lost several hours troubleshooting this.
  • imviewer still fills up inactive memory even with fclose statement and clear statement.  So far no crashes, but output looks questionable — MP02s have hardly any nuclei.  Confirming images on Zen software on lab PC.
  • offer from Janelia (no details), need to arrange second visit.
  • Finish rewriting imviewer_lsm and debugging
  • Run imviewer through recorded data sets
  • MP02 dataset half of embryos start just a bit too deep :(.  Data deleted.
  • Fix embryos (all 7 cages) 1.5-4.5 hr collection, pooled with previous no triton collections.
  • Confocal ON MP10s.
  • new vial of BAC11 early rescues (1 female 2 males).
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