10:40 A -6:30, 8:00P – 8:45P
- continue resin embedding washes. Need light centrifuge to sediment embryos by 1:1 ethanol:resin
- mini-prep BACs grown O/N (16 hrs) in LB-chlor. media. All have very low dirty yield (8-30ng/uL) probably not BAC DNA. BACs too large?
- contact Mounia about DNA isolation kit for BAC DNA.
- Apply to Noah for access to Lichtman group ultratome (in NW building)
- Send CBI imaging registration to Sarah Elwell.
- working on travel grant for Fly meeting
- Submitted application for DeLill Nasser GSA travel gran
- 4:30 p, samples backing O/N at 70 F
- Review house spinning disk confocal operation procedure with Eileen.
- new 5 mL cultures of BACs, including dummy stab.