10:15 A – 6:30 P
- Confocal
- not all embedded samples same color: probably incomplete resin mixing removal of ethanol? not look good.
- Sample labels: 1-3 LamS, 4-6 BcdA, 7-9, BcdS, 10-12 LamA
- New Pacman BAC prep protocol cleaner but even lower yield. 2-4ng/uL, is that even DNA? could try to PCR I suppose.
- Try direct isopropanol precipitation.
- Need to spin down multiple times for clean isolation. Out of cells now. Try again tomorrow, Emi recommends BAC protocol 16.A.
- Order en primers to test DNA isolation.