11:00 A — 9:30P
- Trying Prep 16A for BAC DNA. Isopropanol amounts seem off for volume used, increased to 1.75 mL (50%).
- Prep works fine, conc 380-580 ng/uL. Prepping second half to increase total DNA
- nano-section with Yari (3p – 6:30P)
- Prep buffers for DNA FISH
- Prep more BAC DNA (650-850 ng/uL after -20C precipitation).
- trouble shooting hb-antibody: blocking peptide is only for negative control.