8:30 A — 9:10 P
- Meeting with Bernhard. Probably need to cutaway samples — try 2 micron sections with trimming knife.
- Review dye making with Colenso
- plasticize remaining samples in cylindrical mounts
- nicked translation round 2, try dilution series of DNAse.
- looks like incubation time, not DNAse was the problem. No DNA ladder available in lab to check sizes however and confirm. Stockroom closed. Samples at 4C until tomorrow.
- Learn protocol to rep new slides with Colenso
- Making antibodies with Colenso:
- dk a-m antibody 405:750, 2.6:1.0 (goal 2:1). Conc 2.36 uM
- dk a-rb antibody 405:750, 1.9:0.9. conc 2.68 uM
- Developing new complete fluid exchange embryo wash system (see post).
- antibody stain bcd and hb to test new hb-labeling method from SC — secondary ON at 4C. using new chamber method to improve speed and efficiency of washes.