10:30A — 10:30 P
- prep cover-slips. Need more 22×33 No 1 slides for Hazen.
- Sectioning — 3 embryos — 1st is unfertilized or pre cycle-14, no laminin detected.
- Embryo #2 is cycle 14, very nice laminin. Embryo #3 is very onset of germband elongation stage, also nice laminin stain. Trimmed blocks in eppendorfs.
- 1mmx1mm pyramid blocks work allright, about 1 in 3 settle squarely to the bottom.
- Try pre-selecting embryos and embedding in 2% agarose
- Try using 24 well plates and moving embryos from one to another with a spatula.
- Responses from Fang about image processing code — check tomorrow.
- Lots of colonies from sxl A (5kb), sxl3, sxl1, toll(5kb), y-full, and neu3(5kb) clones (top10 cells split 6 ways). — made new LB-amp and picked colonies.
- Made SOC and pH’d soln. Need to autoclave tomorrow.
- new image simulation with more objects at higher signal clarity finished in 21hrs. Try running on new data tomorrow.