8:50A – 6:10P, 7:10P — 11:30P
- Submit fly abstracts – snail story and super-res imaging methods.
- embedding of H3K27 short primary embryos.
- Shu lab meeting
- Talk to Shu about CUDA implementation of optical section method described in: Mertz2011.
- STORM imaging of multiple sections using Hal, Steve and Dave — took better notes on how to do this process, see post.
- Embedding H3K27 embryos in resin. Transfer from between wells works.
- Can image 647 in resin — easiest to embed in vacuum chambers, sort in chambered slide in resin at the very end, and move from slide into embedding capsule.
- Dissecting scope can see good staging morphology at max mag in resin in chambered slide — good for embryo collection.
- ON primary H3K4, H3K27 and PolII embryos now in 50:50 resin
- H3K27 embryos polyermizing in resin at 70C ON (start 10P).