9:30 A – 8:30P, 10:00P – 11:40P
- Analyzing STORM data on Tuck
- working with inv/en locus data in Matlab
- Embryos in 70%, then pure resin for emedding. Quite slow to drain repeated washes through the membrane filter but still works. Might want a tube splitter to at least process multiple samples in parallel.
- Zeiss confocal time for possible embryo sorting 6p-7p: 647 in these samples doesn’t show up well. Maybe the low conc O/N 4C primary stain (should have directly mounted some embryos).
- Mount short fix H3K27, H3K4, and PolII stained embryos whole-mount on slides
- Flip collection cage 10A-2:30P.
- Fix embryos 3-7 hrs ~wildtype w eyed strain from Kunes
- Make new .1 % GA and 4% PFA 10% .5M EGTA in PBS no tween for ultra-structure fixing.