10:20 A – 10:00P, 10:20P — 12:20A
- in situs day 1: sna[D] 2 vials stained with sna-dig, MP09 2 vials stained with y-full-dig, 1 vial 3-7hr wt E(Pc)-dig, tou-bio, en-dnp. vial 2, 3-7hr wt, en-dig, E(Pc)-bio, tou-dnp.
- don’t remove all of hybe — causes embryos to clump. Cycle in fresh warm hybe from top while removing from below.
- 10.5 hrs of ultratome sectioning, cut 8 coverslips worth of embryo sections. Ribbons work well, moving ribbons still very difficult. designed new large loop probe. Can one pick them up directly onto glass coverslip?
- Overnight imaging on 710. early positions got lost some how? warm-up scan still necessary, haven’t figured out direct focus.