10:45 A – 6:30P, 8:30P – 12:00A
- Overnight confocal imaging failed: did not reactivate 647 and 488 lasers. Did not reset offset to match starting embryo position? Substantial drift still observed without def. focus.
- Staining:
- shp a-dig, rabbit a-bcd,m a-lamin B
- shp a-dig, rabbit a-lamin B, m a-lamin B
- shp a-dig, m a-bio, rab a-lamin B, rab a-H3K27
- Switched embryo containers again, embryos badly ruptured using filters with hybe. Need to run controls to see if its filters + hybe or just full solution change + hybe.