11:00A
- Continuing running O/N snail imaging of 16 wt embryos (finishes about 5P)
- Analyzing snail mRNA counting data, manually checking dot counts.
- offset centroids lead to errors in z intensity (would be better to find the peak value of all adjacent pixels, rather than the one determined as centroid maybe). This causes z-watershed to cleave single dots, which no longer pass the consecutive fields filter.
- Working on snail dynamics figure
- recompute mRNA halflife with error bars
- recompute mRNA synthesis rate with error bars
- Start new O/N confocal imaging of sna-het embryos