10:05 A -1:00 A
- Flip new PcG shRNA lines. Enter into stocks.
- Screen MP12 rescue crosses. set up 6 new crosses, 3 with bright eyes might be homozygous MP12.
- 1 vial with straight wings tossed and 1 vial with Sp tossed.
- Running analysis on en K27 data. Individual section maxes look suggestive.
- Cutting Psc K27 H3 sections.
- Etch Psc 1 hour while shaking in new sodium ethoxide.
- Etching seems to have worked well, will stick with this protocol for 33onm sections for now.
- STORM imaging: 488 conventional looks good, 488 STORM still pretty bad, except for weakly illuminated region on the edge? try dropping laser power?