11:00 A – 8:45 P
- No colonies on plates
- Finish O/N STORM run. Run failed at movie 121, unknown error message.
- attempt bead feducial drift correction:
- polysterene beads good autofluoresnce in all but 750, but bleach fast.
- 405 beads not visible in other channels past 488.
- Bcd/Hb and Ftz stained embryos incubating 1 hr in primary at RT
- image new antibody stains, results recorded in staining record:
- Summary in brief: en, AbdB, and HP1 work after standard fix and MeOH storage.
- Running test gel of PCR linearization of Hox genes and en-locus genes to do new in vitro Tx reactions
- Fly work: flipped all GFP lines into 2 bottles each.
- Fix embryos 15 min, test DSHB antibodies on shorter freshly fixed embryos: m a-Psc, m a-Esc, m a-eve,
- Heat shock transform new cells with old ligation. Mix cells myself, troubleshoot control for KE error.
- Heat fix MP09 embryos (try to match protocol better, have ice at ready).
- Rpt transformations at 37 on partioned plate O/N with IPTG and X-GAL.
- Need to make more amp plates!
Images from antibody testing:
